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Amaxa amaxa 4d nucleofector
Amaxa 4d Nucleofector, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector/4d+amaxa+nucleofector/pmc13253082-82-0-0
Average 86 stars, based on 1 article reviews
amaxa 4d nucleofector - by Bioz Stars, 2026-09
86/100 stars

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Purification:

Article Title: Compositions and methods for enhanced lymphocyte-mediated immunotherapy
Article Snippet: .. Purified CD3 cells stimulated with CD3/CD28 beads were electoporated with recombinant SpCas9 complexed with a chemically modified sgRNA (824) using the amaxa 4D Nucleofector. ..

Article Title: Comprehensive analysis of TEAD inhibition in meningioma identifies MEK and mTOR inhibition as effective combination therapies against resistant lines
Article Snippet: Inhibitors were purchased from MedChemExpress (MCE), Sigma-Aldrich (SA), or SelleckChem (SC) (IAG-933 (MCE HY-153811; SC E1490); MYF-03-176 (SA SML3686-5MG); GNE-7883 (MCE HY-147214), K-975 (MCE HY-138565); trametinib (MCE HY-10999); torin-2 (MCE HY-13002); pictilisib (MCE HY-50094); vistusertib (MCE HY-15247); everolimus (MCE HY-10218); rapamycin (MCE HY-10219); sapanisertib (MCE HY-13328). .. Cells were transiently transfected with ribonucleoprotein complexes composed of chemically synthesized 2′- O -methyl-3′phosphorothioate-modified single guide RNAs (sgRNAs) and purified Cas9 protein using an Amaxa 4D nucleofector as previously described . ..

Recombinant:

Article Title: Compositions and methods for enhanced lymphocyte-mediated immunotherapy
Article Snippet: .. Purified CD3 cells stimulated with CD3/CD28 beads were electoporated with recombinant SpCas9 complexed with a chemically modified sgRNA (824) using the amaxa 4D Nucleofector. ..

Modification:

Article Title: Compositions and methods for enhanced lymphocyte-mediated immunotherapy
Article Snippet: .. Purified CD3 cells stimulated with CD3/CD28 beads were electoporated with recombinant SpCas9 complexed with a chemically modified sgRNA (824) using the amaxa 4D Nucleofector. ..

Transfection:

Article Title: Comprehensive analysis of TEAD inhibition in meningioma identifies MEK and mTOR inhibition as effective combination therapies against resistant lines
Article Snippet: Inhibitors were purchased from MedChemExpress (MCE), Sigma-Aldrich (SA), or SelleckChem (SC) (IAG-933 (MCE HY-153811; SC E1490); MYF-03-176 (SA SML3686-5MG); GNE-7883 (MCE HY-147214), K-975 (MCE HY-138565); trametinib (MCE HY-10999); torin-2 (MCE HY-13002); pictilisib (MCE HY-50094); vistusertib (MCE HY-15247); everolimus (MCE HY-10218); rapamycin (MCE HY-10219); sapanisertib (MCE HY-13328). .. Cells were transiently transfected with ribonucleoprotein complexes composed of chemically synthesized 2′- O -methyl-3′phosphorothioate-modified single guide RNAs (sgRNAs) and purified Cas9 protein using an Amaxa 4D nucleofector as previously described . ..

Article Title: IMP1 is a novel Post-Transcriptional Regulator of Early Erythroid Development and Embryonic Globin output.
Article Snippet: RNPs 150 were prepared by incuba ng 18 pmol Cas9 with 45 pmol synthe c sgRNA (Synthego) for 15 min at 151 25°C. .. Cells (1 × 105) were transfected using an Amaxa® 4D-Nucleofector® (DZ100 program) in P3 NF 152 solu on and recovered in expansion medium for 2–3 days. ..

Synthesized:

Article Title: Comprehensive analysis of TEAD inhibition in meningioma identifies MEK and mTOR inhibition as effective combination therapies against resistant lines
Article Snippet: Inhibitors were purchased from MedChemExpress (MCE), Sigma-Aldrich (SA), or SelleckChem (SC) (IAG-933 (MCE HY-153811; SC E1490); MYF-03-176 (SA SML3686-5MG); GNE-7883 (MCE HY-147214), K-975 (MCE HY-138565); trametinib (MCE HY-10999); torin-2 (MCE HY-13002); pictilisib (MCE HY-50094); vistusertib (MCE HY-15247); everolimus (MCE HY-10218); rapamycin (MCE HY-10219); sapanisertib (MCE HY-13328). .. Cells were transiently transfected with ribonucleoprotein complexes composed of chemically synthesized 2′- O -methyl-3′phosphorothioate-modified single guide RNAs (sgRNAs) and purified Cas9 protein using an Amaxa 4D nucleofector as previously described . ..

other:

Article Title: Dissecting genotype-specific effects of disease-associated genetic variants
Article Snippet: Amaxa 4D Nucleofector , Lonza , N/A.

Electroporation:

Article Title: Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sorting.
Article Snippet: .. Avoid generating air bubbles. k. Place the electroporation cuvette into the slot of the Amaxa 4D Nucleofector. l. Electroporate hiPSCs using the CA137 program. .. Note: As an alternative, CB150 (default program suggested by Lonza for hiPSCs/hESCs) could also be used.



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